Standard Operating Procedures (SOPs)

Cell SOPs: DToxS SOP CE – 1.0: PromoCell Cardiomyocyte Subculture
DToxS SOP CE – 2.0: PromoCell Cardiomyocyte Plating for Drug Test
DToxS SOP CE – 3.0: Preparation of Stocks Solutions for Drug Treatment of Cells
DToxS SOP CE – 4.0: Drug Treatment and Cell Lysis
DToxS SOP CE – 5.0: Healthy Subject Selection
DToxS SOP CE – 6.0: Skin Biopsy Punch Explant Culture for Derivation of Primary Human Fibroblasts
DToxS SOP CE – 7.0: Reprogramming Human Fibroblasts to Human Induced Pluripotent Stem Cells (hiPSCs) using the mRNA with microRNA boost method
Assay SOPs: DToxS SOP A – 1.0: Total RNA Isolation
DToxS SOP A – 2.0: Protein Isolation
DToxS SOP A – 3.0: Quantitative RT-PCR PromoCell Cardiomyocytes
DToxS SOP A – 4.0: Immunofluorescence PromoCell Cardiomyocytes
DToxS SOP A – 5.0: Calcium Dynamics PromoCell Cardiomyocytes
DToxS SOP A – 6.0: High-throughput mRNA Seq Library Construction for 3' Digital Gene Expression (DGE)
DToxS SOP A – 7.0: Sequencing 3'-end Broad-Prepared mRNA Libraries
DToxS SOP A – 8.0: Protein Spectra Acquisition by Data Dependent Acquisition (DDA) on LC/MS/MS
DToxS SOP A – 9.0: Casting of Gels for Microwestern Array
DToxS SOP A – 10.0: Preparation of Adherent Cell Lysates for Microwestern Array
DToxS SOP A – 11.0: Printing of Samples for Microwestern Array
DToxS SOP A – 12.0 HPLC-UV-MS Drug Characterization
DToxS SOP A – 12.0 HPLC-UV-MS Drug Characterization
DToxS SOP A – 13.0: Gel Electrophoresis for Microwestern Array
DToxS SOP A – 14.0: Wet Transfer to Nitrocelluloses for Microwestern Array
DToxS SOP A – 15.0: Antibody Incubation for Microwestern Array
DToxS SOP A – 16.0: Quantifying Microwestern Array Images
Computational SOPs: DToxS SOP CO - 1.0 Protein Identification
DToxS SOP CO – 2.0: Identification of Differentially Expressed Proteins
DToxS SOP CO – 3.1: Generation of Transcript Read Counts
DToxS SOP CO – 4.1: Identification of Differentially Expressed Genes
   
   

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