GAPDH normalized expression of 6 major cardiac genes: sarcomeric actinin, cardiac troponin T, HERG channel, and the 3 major cardiac associated transcription factors. Expression has been quantified with RT-PCR. These markers have been associated with cardiac differentiation; their upregulation demostrate diffentiation of this cell line.
| PromoCell Cardiomyocyte, Line D Assessment of differentiation markers by qRT-PCR |
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|---|---|
| Assay Date(s): | 02/26, 03/05, 06/23 |
| Raw Data: | Raw cycle counts |
| Related Standard Operating Procedures (SOPs) | |
| Cell SOPs: | DToxS SOP CE – 1.0: PromoCell Cardiomyocyte Subculture |
| DToxS SOP CE – 2.0: PromoCell Cardiomyocyte Plating for Drug Test | |
| DToxS SOP CE – 4.0: Drug Treatment and Cell Lysis | |
| Assay SOPs: | DToxS SOP A – 1.0: Total RNA Isolation |
| DToxS SOP A – 3.0: Quantitative RT-PCR PromoCell Cardiomyocytes | |