Intracellular calcium trace from a representative PromoCell cardiomyocyte (Line B) that shows no intracellular calcium cycling after 4 weeks of differentiation. Red pulses represent the 10 ms 40 V pulse stimulation.
| Assay Date(s): | 07/22/2015 |
| Sample Size: | 30 |
| Dye Platform: | Rhod-2 AM (5 uM, 30 min) |
| Temperature: | 37ºC |
| Imaging Platform: | Zeiss LSM 510 |
| Excitation: | 543 nm HeNe laser |
| Emission: | 560 nm (longpass) |
| Electrodes: | 0.5 mm |
| Solution Concentration in mM | |
| NaCl: | 140 |
| KCl: | 5.4 |
| CaCl2: | 2 |
| MgCl: | 1 |
| HEPES: | 5 |
| Glucose: | 10 |
| pH: | 7.4 (NaOH) |
| Number of Cells per Different Activity Type | None or random fluctuations: | 30 |
| Delayed/slow increase with stimulation: | 0 |
| Immediate increase with stimulation: | 0 |
| Related Standard Operating Procedures (SOPs) | |
| Cell SOPs: | DToxS SOP CE – 1.0: PromoCell Cardiomyocyte Subculture |
| DToxS SOP CE – 2.0: PromoCell Cardiomyocyte Plating for Drug Test | |
| DToxS SOP CE – 4.0: Drug Treatment and Cell Lysis | |
| Assay SOPs: | DToxS SOP CE – 5.0 Immunofluorescence PromoCell Cardiomyocytes |
| Performed by: | B. Hu, R. A. Devenyi |