Intracellular calcium trace from a representative PromoCell cardiomyocyte (Line B) that shows no intracellular calcium cycling after 4 weeks of differentiation. Red pulses represent the 10 ms 40 V pulse stimulation.
Assay Date(s): | 07/22/2015 |
Sample Size: | 30 |
Dye Platform: | Rhod-2 AM (5 uM, 30 min) |
Temperature: | 37ºC |
Imaging Platform: | Zeiss LSM 510 |
Excitation: | 543 nm HeNe laser |
Emission: | 560 nm (longpass) |
Electrodes: | 0.5 mm |
Solution Concentration in mM | |
NaCl: | 140 |
KCl: | 5.4 |
CaCl2: | 2 |
MgCl: | 1 |
HEPES: | 5 |
Glucose: | 10 |
pH: | 7.4 (NaOH) |
Number of Cells per Different Activity Type | None or random fluctuations: | 30 |
Delayed/slow increase with stimulation: | 0 |
Immediate increase with stimulation: | 0 |
Related Standard Operating Procedures (SOPs) | |
Cell SOPs: | DToxS SOP CE – 1.0: PromoCell Cardiomyocyte Subculture |
DToxS SOP CE – 2.0: PromoCell Cardiomyocyte Plating for Drug Test | |
DToxS SOP CE – 4.0: Drug Treatment and Cell Lysis | |
Assay SOPs: | DToxS SOP CE – 5.0 Immunofluorescence PromoCell Cardiomyocytes |
Performed by: | B. Hu, R. A. Devenyi |